They made a PNP hydrogel formulation of desired final concentration by mixing the stock solutions of dodecyl-modified (hydroxypropyl)methylcellulose (HPMC-C 12) and polyethylene glycol–polylactic acid (PEG-PLA) nanoparticles in the requisite amount of phosphate buffer solution (PBS). In the present study, researchers explored materials that could form a subcutaneous depot and successfully deliver antibody therapeutics with different PK characteristics. Addressing the challenge of bnAbs burdensome administration could also improve pre-and post-exposure prophylaxis of bnAbs against SARS-CoV-2. A poor PK profile depletes the concentration of bnAbs in the body, thereby hampering its prophylactic or therapeutic effectiveness. The persistent protective action of bnAbs could enable passive immunization against infectious diseases, including coronavirus disease 2019 (COVID-19). It readily prevents the burst release of encapsulated bnAbs and slowly releases the antibody-drug cargo to improve its pharmacokinetics (PK). PNP hydrogel is a supramolecular hydrogel with shear-thinning and self-healing properties. Image Credit: Corona Borealis Studio/Shutterstock Background Study: Subcutaneous delivery of an antibody against SARS-CoV-2 from a supramolecular hydrogel depot. In a recent study posted to the bioRxiv* pre-print server, researchers compared the polymer-nanoparticle (PNP) hydrogel system and standard routes of delivery of broadly neutralizing antibodies (bnAbs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a mouse model. Gertrude ends the letter telling not to expect any correspondence after such and said, "this is my final epistle.By Neha Mathur Reviewed by Aimee Molineux Gertrude witnessed the Hyperborea explode and she was taken by the Ghastlyhaunts. That leaves Gertrude who witnesses the Hyperborea approaching its target and the base of Disparate protected with a Dyson sphere, seeing the power of Disparate and the uselessness of the struggles they have been through. X arrives and Alex knows her from when he was young, they both decided to leave reality. Meanwhile, they were trying to figure out what to do with the Hyperborea and they cannot seem to agree with each other as Maas wanted the ship to be handed to the Resistance for further research while Alex wanted to end it as Father Eli wished.Īlex turned against Maas killing her and proceeded to destroy the Hyperborea, the heart of the invasion plot of Disparate. Together, they were able to witness an epic moment, the Seven Hour War. Bree, a slug-creature who has the consciousness of a person, who asked Alex and Gertrude to end his life.Īlex and Gertrude also had a prison break to rescue Dr.
Hyperborea appears and disappears through time and space and whenever it is present, Disparate studies it. The story centers on the search for Hyperborea which is surrounded by Disparate's base. X (the G-Man), Disparate (Combine), and Dr. While in the journey, Gertrude and Alex came across Mrs. Laidlaw shared on his personal Twitter account that the story is "a genderswapped snapshot of a dream I had many years ago." He also hinted that he was "just planting seeds" as regards the relationship of Alex Vance and G-Man.Įpistle 3 is a letter of Gertrude Fremont addressed to "Playa" which goes around the story of how after the death of Elly Vaunt, Gertrude Fremont and Alex Vance made their way to the Antarctic to look for the ship called the Hyperborea (Borealis), the vessel revealed in Half-Life 2: Episode 2 where research takes place. Epistle 3 details appear to be in consonance with the leaks and rumors surrounding Half-Life 2: Episode Three which is set in the Antarctic where Gordon and Alex are found near a helicopter that crashed.